Cellular uptake of ribonuclease A-functionalised core–shell silica microspheres
نویسندگان
چکیده
منابع مشابه
Cellular uptake of ribonuclease A relies on anionic glycans.
Bovine pancreatic ribonuclease (RNase A) can enter human cells, even though it lacks a cognate cell-surface receptor protein. Here, we report on the biochemical basis for its cellular uptake. Analyses in vitro and in cellulo revealed that RNase A interacts tightly with abundant cell-surface proteoglycans containing glycosaminoglycans, such as heparan sulfate and chondroitin sulfate, as well as ...
متن کاملOn-demand cellular uptake of cysteine conjugated gadolinium based mesoporous silica nanoparticle with breast cancer-cells
Design, synthesis, and conjugation of mesoporous silica nanoparticles (MSNs) with biomolecules is a matter of growing interest to enhance selective uptake of contrast agents like gadolinium (Gd3+) by cancer cells. Here, by targeting xc-cystine/glutamate antiporter system in breast cancer cells, conjugation of MSN-Gd3+ with cysteine is used to enhance cancer cellular uptake of Gd3+. Reactions de...
متن کاملFrictional performance of silica microspheres
Wepresent a fundamental study of the frictional performance of silicamicrospheres, either 4, 2, or 0.5 mm in diameter, on a silicon substrate. The tribological properties of these rolling systems were measured with a ball-on-flat tribometer at various loads. The frictional performance of the rolling systems is found to be highly dependent on load and sphere diameter. Rolling motion was confirme...
متن کاملSilica-Based Nanoparticle Uptake and Cellular Response by Primary Microglia
BACKGROUND Silica nanoparticles (SiNPs) are being formulated for cellular imaging and for nonviral gene delivery in the central nervous system (CNS), but it is unclear what potential effects SiNPs can elicit once they enter the CNS. As the resident macrophages of the CNS, microglia are the cells most likely to respond to SiNP entry into the brain. Upon activation, they are capable of undergoing...
متن کاملBiomimetic silica microspheres in biosensing.
Lipid vesicles spontaneously fuse and assemble into a lipid bilayer on planar or spherical silica surfaces and other substrates. The supported lipid bilayers (SLBs) maintain characteristics of biological membranes, and are thus considered to be biomembrane mimetic systems that are stable because of the underlying substrate. Examples of their shared characteristics with biomembranes include late...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: J. Mater. Chem. B
سال: 2014
ISSN: 2050-750X,2050-7518
DOI: 10.1039/c4tb01130a